Determination of the number of proteins in liver ribosomes and ribosomal subunits by two-dimensional polyacrylamide gel electrophoresis.

نویسندگان

  • C C Sherton
  • I G Wool
چکیده

The number of proteins in rat liver ribosomes was determined by two-dimensional polyacrylamide gel electrophoresis. There were 30 proteins in the 40 S subunit, 39 in the 60 S subunit. The proteins of the two subunits, with one possible exception, are unique. Preparations of 80 S ribosomes contained three proteins not present in either subunit; thus, we estimate that eukaryotic ribosomes contain between 68 and 72 different proteins.

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عنوان ژورنال:
  • The Journal of biological chemistry

دوره 247 14  شماره 

صفحات  -

تاریخ انتشار 1972